Unit BIOSENSORS AND BIOMATERIALS

Course
Biotechnology
Study-unit Code
A003034
Curriculum
In all curricula
Teacher
Benedetta Carlotti
Teachers
  • Benedetta Carlotti
Hours
  • 47 ore - Benedetta Carlotti
CFU
6
Course Regulation
Coorte 2022
Offered
2024/25
Learning activities
Affine/integrativa
Area
Attività formative affini o integrative
Academic discipline
CHIM/02
Type of study-unit
Obbligatorio (Required)
Type of learning activities
Attività formativa monodisciplinare
Language of instruction
English
Contents
Introduction to fluorescence. Use of fluorescent sensors to probe pH, ions, cellular polarity and viscosity, oxygen reactive species, membrane potentials. Use of fluorescent sensors for the specific investigation of different biomaterials. Photodynamic therapy of tumors.
Reference texts
David M. Jameson, INTRODUCTION TO FLUORESCENCE, 2014, CRC Press.
Iain D. Johnson, MOLECULAR PROBES HANDBOOK-A GUIDE TO FLUORESCENT PROBES AND LABELING TECHNOLOGIES, 2010, InVitrogen.
Educational objectives
The student will become familiar with the most important concepts about the choice and use of fluorescent sensors for the investigation of biomaterials.
Prerequisites
In order to understand the topics covered in the course, basic knowledge of Molecular Spectroscopy and Chemical Kinetics are required. These issues will be addressed in some lessons that will be held at the beginning of the course.
Teaching methods
Lectures with slide projections. Some lessons will be dedicated to the deepening of the addressed issues, some others to the visit of laboratories of our department.
Other information
Attending the lectures is not compulsory but strongly recommended. Tutoring activity is planned during the course and for the students who request it to assist them in the preparation of the exam.
Learning verification modality
The exam consists in an oral test. The oral examination is a discussion lasting about 30 minutes aimed at ascertaining the level of knowledge and understanding reached by the student on the topics listed in the program. The oral exam will also serve to verify the communication skills of the student and ability in autonomous organization of the exposition.
Extended program
Introduction to fluorescence. Light absorption. Instrumentation for fluorescence measurements. Time resolved fluorescence. Anisotropy. FRET. Fluorescence microscopy. Fluorescent sensors to probe pH, ions, cellular polarity and viscosity, oxygen reactive species, membrane potentials. Conjugation of fluorescent sensors to biomolecules: amino reactive probes, thiol reactive probes. Probes for different biomolecules and cellular compartments. Fluorescent proteins. Photodynamic therapy of tumors.
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