Unit BIOMEDICAL VISION LABORATORY
- Course
- Optics and optometry
- Study-unit Code
- A002511
- Location
- TERNI
- Curriculum
- Optometria
- Teacher
- Marina Maria Bellet
- Teachers
-
- Marina Maria Bellet
- Hours
- 47 ore - Marina Maria Bellet
- CFU
- 6
- Course Regulation
- Coorte 2022
- Offered
- 2023/24
- Learning activities
- Affine/integrativa
- Area
- Attività formative affini o integrative
- Academic discipline
- MED/46
- Type of study-unit
- Opzionale (Optional)
- Type of learning activities
- Attività formativa monodisciplinare
- Language of instruction
- Italian
- Contents
- Knowledge of the scientific method and guidelines of good clinical and laboratory practice (GCP and GCLP); knowledge of the main fields of instrumental and laboratory diagnostics relating to diseases associated with vision. Main techniques and methods of research laboratories applied to otpometric studies. Animal and cellular experimental models.
- Reference texts
- - Mauro Macarrone. Metodologie biochimiche e biomolecolari. Zanichelli 1° Edizione.
Support material projected during the lessons in electronic format will be available on Unistudium. - Educational objectives
- To acquire knowledge and laboratory diagnostic techniques for the pathologies responsible for vision alterations. Ability to understand the scientific method and research protocols and study designs.
- Prerequisites
- In order to understand and to know to tackle the majority of the information given within the Course, you must have basic knowledge of Biology.
- Teaching methods
- Face-to-face lectures with audiovisual material on all subjects of the Course.
- Learning verification modality
- The exam consists of an oral test with a duration depending on the performance of the test itself (about 20 minutes, 2 questions). The test aims at evaluate the level of knowledge and understanding achieved by the student. In particular, it will be evaluated the acquisition of knowledges relatively to the main experimental laboratory activities in both diagnostic and research fields (1st question) and their applications in the field of vision pathologies (2nd question).
For information on support services for students with disabilities and / or SLD, visit the page
http://www.unipg.it/disabilita-e-dsa - Extended program
- - Introduction to the biomedical laboratory: use of basic instrumentation in the laboratory
- Main laboratory techniques: centrifuge and spectroscopic techniques, chromatographic and electrophoretic techniques, radio and immunochemical techniques
- Diagnostics in the laboratory, GCP and GCLP. The eye in systemic diseases.
- Microbiological laboratory methods: diagnostics of eye infections
- Laboratory methodologies in Molecular Biology: isolation of DNA and RNA, PCR amplification techniques, expression vectors and molecular cloning. Molecular diagnostics in the eye, gene therapy of eye diseases.
- Laboratory methodologies in Cell Biology: cell cultures, organoids, cell transfection, silencing and overexpression, CRISPR/CAS9 technology. Cell lines and ocular organoids, application of CRISPR-CAS9 to ocular pathologies.
- Omic technologies: Transcriptomics and bioinformatics: the human eye transcriptome atlas. Proteomics and metabolomics: ocular biomarkers
- Epigenetics and its applications. Epigenetics in ocular diseases
- Histological preparations and microscopy. Histopathology of the ocular surface
- Animal models for in vivo experiments. Mouse models of eye diseases
- From data analysis to the publication of a scientific work. Study of selected publications.