Unit ANIMAL REPRODUCTION BIOTECHNOLOGY

Course
Medical, veterinary and forensic biotechnological sciences
Study-unit Code
A001780
Curriculum
Veterinario
Teacher
Riccardo Zelli
Teachers
  • Riccardo Zelli
Hours
  • 52 ore - Riccardo Zelli
CFU
6
Course Regulation
Coorte 2024
Offered
2025/26
Learning activities
Caratterizzante
Area
Discipline veterinarie e riproduzione animale
Sector
VET/10
Type of study-unit
Opzionale (Optional)
Type of learning activities
Attività formativa monodisciplinare
Language of instruction
English
Contents
Main aspects of the physiology of reproduction in animals. Heat synchronization and embryo transfer. In vitro fertilization of egg cells. Thecniques of seminal collection, evaluation end conservation (n.13 hours) Andrological evaluation: Direct clinical and instrumental examinations, laboratory methods for seminal analysis ( No. 5 hours). Infertility and reproductive diseases: etiopathogenesis, diagnosis and treatment: impotentia coeundi; Impotentia generandi; Hereditary and acquired disorders of the testis, epididymis and accessory sex glands ( N. 5 hrs.) Diagnosis and treatment of abnormalities of the genital system in different species ( No. 5 hours) Practical lesson: 24 hours
Reference texts
Reproduction in farm animals. 7th edition. E. S. E. Hafez (Editor), B. Hafez (Editor) Pathways to Pregnancy and Parturition. by P.L. Senger
Educational objectives
Provide students with the basic knowledge of the physiology of reproduction in domestic animals, embryo transfer techniques, in vitro fertilization of oocytes, collection, evaluation and conservation of semen material. D1 - KNOWLEDGE AND UNDERSTANDING The student must have: 1. Knowledge of reproductive physiology of animals of zootechnical interest 2. Knowledge of the methods of collection, analysis and conservation of semen 3. Knowledge of the clinical bases to improve reproductive performance 4. Knowledge of reproductive pathologies 5. Knowledge of reproductive hygiene D2 - ABILITY TO APPLY KNOWLEDGE AND UNDERSTANDING At the end of the training activity, the student must be able to: 1. evaluation and conservation of semen 2. embryo transfer techniques and in vitro fertilization of oocytes D3 - JUDGMENT ABILITY At the end of the training activity, the student must be able to: 1. Evaluate the potential fertility of the various domestic animal species 2. apply the various reproductive biotechnology techniques in the different species D4 - SKILLS COMMUNICATION At the end of the training activity, the student must be able to: 1. organize, prepare and present, to an audience composed of people with the same level of preparation, a presentation on a specific reproductive technique with their own evaluations supported by appropriate arguments, 2. sustain an open debate with people with the same preparation and experts in different topics, of a regulatory, scientific, procedural and/or technological nature, 3. demonstrate command of language in both written and oral form, as well as the ability to use sufficiently appropriate terminology for a correct approach to the profession, also important for job interviews. D5 - LEARNING ABILITY At the end of the training activity, the student must be able to: 1. consult and understand scientific texts, including innovative ones, bibliographic updates, regulatory dictates, in such a way as to use them in contexts that are not only usual for the profession, including research, but also original, 2. possess a sufficiently broad mastery of the subject to guarantee an acceptable basis for continuing professional development throughout life, through permanent continuous training.
Prerequisites
The student, in order to understandand how to apply most of the reproductive techniques of the dog,the cat and of livestock. In addition, other topics covered in the module require the ability to interpret the various tests such as the ultrasonography and endoscopic of reproductive tract in different species, endocrinological examinations, vaginal citology and spermiograms. The knowledge of the above is a prerequisite for the student who wants to follow the course with profit.
Teaching methods
Teoretical lessons will be carried out in the classroom, while the practices at the laboratories of the Department of Veterinary Medicine
Other information
The lecture will be held at the OVUD and the teaching center of the Department of Veterinary Medicine. For the calendar of activities, see the website on page Lectureshttp://www.med.unipg.it/ccl/Biotechnological_Sciences/Teaching_time_Table/
Learning verification modality
Oral exam
Extended program
Outlines of the anatomy of the male and female reproductive system. - Reproductive endocrinology: hypothalamic-pituitary-gonadal axis. Pituitary gonadotropins, steroid hormones. - Reproductive physiology: oogenesis and folliculogenesis. Ovulation and corpus luteum formation. Spermatogenesis. Preimplantation embryonic development. Reproductive cycle: estrous cycles and menstrual cycle. -Hormonal control of the ovarian cycle: Synchronization and superovulation techniques, artificial insemination (AI) and embryo transfer (ET). -Overview of 1st, 2nd and 3rd level PMA techniques. -Assisted reproductive techniques laboratory set-up: equipment and materials. -Ovum pick-up (OPU). Oocyte pick-up of ovaries from slaughter or ovariectomy sites. Qualitative evaluation of oocytes, set-up of in vitro maturation (IVM) systems. -Collection, evaluation and preparation of semen. Setting up systems for in vitro fertilization (IVF, IVF). Intracytoplasmic sperm injection (ICSI). - Evaluation of early stages of embryo development and embryo quality. Setup of in vitro embryo culture (IVC) systems. -Micromanipulation of oocytes and embryos; embryo splitting; cloning by nuclear transfer. -Cryopreservation of gametes, embryos and ovarian tissue. Freezing and vitrification.
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