Unit GENERAL APPLIED AND CLINICAL BIOCHEMISTRY
- Course
- Industrial pharmacy
- Study-unit Code
- A003594
- Curriculum
- In all curricula
- Teacher
- Carmela Conte
- Teachers
-
- Carmela Conte
- Michela Codini (Codocenza)
- Hours
- 51 ore - Carmela Conte
- 36 ore (Codocenza) - Michela Codini
- CFU
- 10
- Course Regulation
- Coorte 2024
- Offered
- 2025/26
- Learning activities
- Caratterizzante
- Area
- Discipline biologiche e farmacologiche
- Sector
- BIO/10
- Type of study-unit
- Obbligatorio (Required)
- Type of learning activities
- Attività formativa monodisciplinare
- Language of instruction
- Italian
- Contents
- Knowledge of the main biological macromolecules and the relationship between structure and function.
Metabolisms and related clinical aspects. Main biochemical methods for the study of macromolecules. - Reference texts
- - I principi di Biochimica di Lehninger Di
Nelson and Cox- Zanichelli Editore
- Biochimica Medica di Siliprandi e Tettamanti, Piccin editore. - Educational objectives
- At the end of the study the student will be able to acquire the basic knowledge of the fundamental principles of General, applied and clinical biochemistry.
- Prerequisites
- The course of general, applied and clinical biochemistry aims to address topics that the student will be able to learn if he has acquired the basic knowledge of inorganic and organic chemistry. In order to understand the contents of the course, it is essential that the student has acquired the fundamental notions on the structure of the main macromolecules and chemical bonds as well as their behavior during metabolic reactions.
- Teaching methods
- Teaching will be carried out through lectures in the classroom covering all the topics of the program and exercises for the applied biochemistry part which will take place in the laboratory.
- Other information
- none
- Learning verification modality
- The evaluation includes a single oral test during which the student will have to answer questions regarding the topics covered during the lessons and exercises. During the interview the student will have to demonstrate coherence with the proposed question, clarity of presentation and adequate scientific terminology
- Extended program
- * Introduction to the Biochemistry.
* Water and weak interactions in aqueous systems. Ionization of water, weak acids, and weak bases. Biological buffers.
* Fundamental thermodynamic concepts.
* Structure and function of the main biomolecules.
* Nucleic acids: Nucleotides. DNA and RNA structures and main functions.
* Cell membranes and transmembrane transport.
* Carbohydrates: monosaccharides, disaccharides and polysaccharides and their implications in human biochemistry.
* Glucose metabolism: Glycolysis, Glycogenolysis, Gluconeogenesis, Pentose phosphate shunt.
* Lipids: the main classes of lipids and their implications in human biochemistry. Fatty acids, acylglycerols, phospholipids, sphingolipids, glycolipids, and polyprenoids. Cholesterol. Lipids as signals and cofactors.
* Lipid metabolism: Fatty acid catabolism, Ketone bodies, lipogenesis.
* Electron transport and oxidative phosphorylation
* Amino acids. Classification and description of the twenty alpha-L-amino acids that constitute the proteins.
* Proteins: structures and functions. Primary, Secondary, tertiary, and quaternary structure. Globular and fibrous proteins. Alpha-keratins and fibroin, collagen.
* Amino acid metabolism. Fate of the amino group. Glucogenic and ketogenic amino acids. Transamination. Urea cycle
* Protein folding.
* Myoglobin and hemoglobin; relationships between structure and function * Vitamins and Biochemical action of vitamins. Coenzymes: structure and functions.
* Enzymes: General classification, catalytic activity and structure; cofactors and prosthetic groups; Enzyme kinetics. Michaelis and Menten model. Km and Vmax values. Competitive, non-competitive and irreversible inhibition. Mechanisms of enzymatic catalysis.
* Enzyme regulation. Allosteric enzymes and Kinetics. Covalent regulation: reversible and irreversible.
* Signal Transduction.
* Methods for protein isolation and study. - Obiettivi Agenda 2030 per lo sviluppo sostenibile
- health and wellness