Unit IMMUNOLOGICAL BIOTECHNOLOGY
- Course
- Molecular and industrial biotechnology
- Study-unit Code
- 55A02006
- Curriculum
- In all curricula
- Teacher
- Francesco Grignani
- Teachers
-
- Francesco Grignani
- Hours
- 52 ore - Francesco Grignani
- CFU
- 6
- Course Regulation
- Coorte 2021
- Offered
- 2021/22
- Learning activities
- Caratterizzante
- Area
- Discipline per le competenze professionali
- Academic discipline
- MED/04
- Type of study-unit
- Obbligatorio (Required)
- Type of learning activities
- Attività formativa monodisciplinare
- Language of instruction
- Italian
- Contents
- Biotechnologies using immunological tools in the study, diagnosis and therapy of human pathology, with a selection of the most advanced immunological, cellular and molecular tools.
- Reference texts
- Janeway's Immunobiology
Garland Science
Abbas, Lichtman, Pillai:
Cellular and Molecular Immunology - Educational objectives
- Basic knowledge of the immune system and the main immunological approaches in the biotechnological sector. In particular, knowledge of classic and modern polyclonal and monoclonal antibody production strategies; on the diagnostic and therapeutic applications of antibodies in oncology, rheumatology and cardiology; on the fundamental immunochemical and immunofluorescence techniques, including the flow cytometer; on the main applications in research of Immunoprecipitation technologies for the analysis of proteins, RNA, and chromatin.
- Prerequisites
- Cell biology and signaling transduction. Innate and specific immune system cells. Basic mechanisms of immune reactivity.
- Teaching methods
- Lectures, class discussions, visualization and commentary of images and laboratory procedures. Viewing movies. Work in a group.
Inverted classes.
Peer to peer teaching - Learning verification modality
- Classroom discussion. Presentation of specific topics and insights and Bibliographic research, as oral exam constituents
- Extended program
- 2576/5000
- Basic immunology: innate and acquired immunity. Antigens and antigenic recognition by B and T lymphocytes. The antibody response. I-IV hypersensitivity reactions such as allergies and diagnostic tests.
- Structure and function of antibodies (Ab) or immunoglobulins (Ig).
- Protein immunoprecipitation: principles and uses.
- Immunological assays and methods based on the antigen-antibody reaction: precipitation (in solution and agarose gel) and agglutination. Some applications in the biomedical diagnostic field: blood typing, bacterial typing, pregnancy test, Coombs test. Nephelometry, Turbidimetry
- Polyclonal antibodies: production of an antiserum and its applications.
- Monoclonal antibodies (mAb): production of murine, chimeric, humanized, human mAb (using phage display technology, transgenic animals and other technologies).
- Applications of mAb in the diagnostic and therapeutic biomedical field.
- Mechanisms of therapeutic antibody activity
- Bispecific antibodies, BITEs and Single Chain Variable Fragments (scFv)
- Chimeric antigenic receptors (CARs)
- Antibodies used in anti-inflammatory therapy and in immune pathologies
- Antibodies used in cholesterol lowering therapy
- Immune response to tumors and tumor immunotherapy.
- Signal transduction of growth factors and main types of receptors.
- Antibodies active on proliferative and angiogenetic signaling
- Antibodies useful for activating anti-tumor immunity (CTLA-4 system, PD-1 PDL, others)
- Cellular immunotherapy and CAR-Ts
- Immunotherapeutic combinations.
- Chromatin structure and epigenetic regulation of gene expression
- Chromatin Immunoprecipitation (ChIP)
- ChIP Sequencing and ChIP on chip
- RNA immunoprecipitation (RIP)
- RIP-Sequencing
- Direct and indirect immunofluorescence techniques for the identification of surface and intracellular antigens.
- Flow cytometry. Its applications in the analysis of the normal and pathological white blood cell phenotype. Elements of cell sorting.
- Radio Immunological Assays (RIA, radio immuno-assay) and Immuno-Enzyme Adsorption ELISA (enzyme-linked immunosorbent assay), for the identification and dosage of antigens and antibodies. Some applications in the biomedical diagnostic field
- Transplants and transplant rejection: tissue typing and donor-recipient compatibility tests.
- Immune response to infections and strategies for vaccine development.