Unit CELLULAR AND TISSUE BIOTECHNOLOGY
- Course
- Molecular and industrial biotechnology
- Study-unit Code
- 55A01906
- Curriculum
- In all curricula
- Teacher
- Mario Calvitti
- Teachers
-
- Mario Calvitti
- Hours
- 52 ore - Mario Calvitti
- CFU
- 6
- Course Regulation
- Coorte 2021
- Offered
- 2021/22
- Learning activities
- Affine/integrativa
- Area
- Attività formative affini o integrative
- Academic discipline
- BIO/17
- Type of study-unit
- Opzionale (Optional)
- Type of learning activities
- Attività formativa monodisciplinare
- Language of instruction
- Italian
- Contents
- Morphological features of human normal tissues. Staining methods - Histologic and Histochemical. Cell culture.
- Reference texts
- - Istologia per le Professioni Sanitarie, Sica et al. Casa Editrice Idelson-Gnocchi, 2015.- M. Melis-F. Carpino-U. Di Tondo. Tecniche in Anatomia Patologica - edi-ermes.- E. Bonucci – “Manuale di Istochimica”- Edi-Lombardo.- Anatomia Patologica – Le basi – Luigi Ruco, Aldo Scarpa – UTET
- Educational objectives
- At the end of the course, student must reach the critical knowledge of:
A) morphological features of human normal tissues.
B) Staining methods - Histologic and Histochemical
C) Cell culture. - Prerequisites
- In order to be able to understand and apply the majority of the contents described within the course, the student must have acquired basic knowledge of cellular biology.
- Teaching methods
- Face-to-face.
Practical training
Theoretical lessons and practical training
Laboratory. - Other information
- Recommend the attendance.
- Learning verification modality
- At the end of the course, student must reach the critical knowledge of:
A) morphological features of human normal tissues.
B) Staining methods - Histologic and Histochemical
C) Cell culture. - Extended program
- Course of Histology. Lining epithelium: general considerations, classification and main examples. Exocrine and endocrine glands: classification and examples.
Connective tissues and adipose tissue. Cartilage. Bone tissue. Bone development. Blood (plasma; erythrocytes, granulocytes, lymphocytes, monocytes, platelets).
Muscular tissues: morphology of smooth and striated muscles. Molecular mechanism of contraction. Nervous tissue: general organization. Neurons and neuroglial cells.
Nerve fibers, peripheral nerves. Synapses: morpho-functional organization. Staining methods - Histologic and Histochemical. Tissue fixation. Aldehyde fixative. Formalin. Glutaraldehyde. Formaldehyde. Bouin's fluid. Osmium tetroxide as fixative. Decalcification of bone. Dehydration. Paraffin embedding. Tissue Sectioning. Microtome with paraffin section. Cold microtome (cryostat) methods. Microtome with frozen section. Frozen section routines. Paraffin section methods. Deparaffinization. Treatment of section before and after staining. Dehydration of paraffin section. Mounting of sections. Ematoxylin and Eosin for paraffin section. Azan-Mallory. Alcian blu-haematoxylin staining. Alcian blue 8GX for acid mucopolysaccharides. The Periodic Acid-Schiff Reaction (PAS). PAS- haematoxylin staining. Alcian blue-PAS stain. Toluidine blue stains for metachromasia. Resorcin fuchsin stain of Weigert. Weigert for elastic fiber stain. Von Kossa method for phosphates and carbonates. Silver impregnation method for reticulum fibers. Sudan III. Immunofluorescence. Electron microscopy.
Cell culture. Isolation of cells. Maintaining cells in culture. Manipulation of cultured cells. Media changes. Passaging cells. Applications of cell culture.