Unit CHEMISTRY AND BIOCHEMISTRY I

BIOCHEMISTRY I - MOD. 1

Code	GP001280
Location	PERUGIA
CFU	3
Teacher	Ilaria Bellezza
Teachers	Ilaria Bellezza
Hours	37.5 ore - Ilaria Bellezza
Learning activities	Base
Area	Struttura, funzione e metabolismo delle molecole d'interesse biologico
Academic discipline	BIO/10
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Structural biochemistry: carbohydrates, lipids, nucleotides, nucleic acids, amino acids, proteins. Hemoglobin and myoglobin
Reference texts	I principi di Biochimica del Lehninger - Nelson, Cox, Zanichelli Harper's Biochimica Illustrata - Murray et al - EMSI Biochimica Umana Salvatore Idelson Gnocchi Biochimica Devlin EdiSES Biochimica Berg, Tymoczko, Stryer Zanichelli Biochimica Medica Siliprandi, Tettamanti Piccin
Educational objectives	The main competence will be to have a general knowledge necessary to address the lessons of Module 2.
Prerequisites	The student must have acquired basic knowledge of the main topics of General Chemistry and Organic Chemistry
Teaching methods	The course is organized in:-frontal lessons-theoretical practical lessons
Other information	The attendance is compulsory at least 75% of lessons. Perugia –School of Medicine – Educational and Scientific Center
Learning verification modality	The final exam consists of a written test with multiple choice questions and an oral exam
Extended program	Nucleotides and Nucleic acids: purine and pyrimidine bases, nucleosides, nucleotides, DNA and RNA. Carbohydrates: monosaccharides, disaccharides, polysaccharides. Lipids: Fatty Acids, Triglycerides, Phospholipids and Glycolipids, Cholesterol. Amino acids and proteins: acid base properties. Peptide bonding. Fibrous and globular proteins. Primary, secondary, tertiary and quaternary structure of proteins. Isoelectric point. Biological functions. Structure and function of biological membranes: composition of biological membranes. Structure and properties of membrane lipids. Structure and properties of membrane proteins. Transport systems across the membrane. Chemical mediators, receptors and signal transduction. Generalities on primary and secondary chemical mediators. Structure and properties of some chemical mediators. Properties of receptors and their localisation. Signal transduction across the plasma membrane. Regulation of adenylate cyclase. cGMP and nitric oxide. Phosphoinositide cycle. Regulation of intracellular calcium concentration. Modulation of enzyme activities by phosphorylation-dephosphorylation reactions. Protein kinases. Induction of protein expression.

BIOCHEMISTRY I - MOD. 2

Code	GP001282
Location	PERUGIA
CFU	3
Teacher	Davide Chiasserini
Teachers	Davide Chiasserini
Hours	37.5 ore - Davide Chiasserini
Learning activities	Base
Area	Struttura, funzione e metabolismo delle molecole d'interesse biologico
Academic discipline	BIO/10
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	The Module 2 program covers the following topics: Enzymes. Coenzyme structure and action mechanism. Catabolism and Anabolism. Bioenergetics. High energy bonds. ATP. Pyruvate dehydrogenase. Cycle do Krebs. Respiratory chain and oxidative phosphorylation
Reference texts	To be defined
Educational objectives	At the end of the course the student should be in possession of the main knowledge related to Structural Biochemistry, Catabolism and Anabolism and Energy Biochemistry
Prerequisites	The student must have acquired basic knowledge of the main topics of General Chemistry and Organic Chemistry and Structural Biochemistry
Teaching methods	The course is organized in:-frontal lessons-theoretical practical lessons
Other information	The attendance is compulsory at least 75% of lessons. Perugia –School of Medicine – Educational and Scientific Center
Learning verification modality	The final exam consists of a written test with multiple choice questions and an oral exam
Extended program	Enzymes: enzyme catalysis. Active site. Specificity. Nature of the Enzyme and their classification. Enzymes constitutive and inductive. Kinetics of enzymatic reactions. Michaelis-Menten constant and its meaning. Factors affecting the activity of enzymes. Enzyme inhibition. Mechanisms of enzyme inhibition. regulation of enzyme activity. signs of clinical enzymology. Vitamins and coenzymes: food sources of vitamins. Determination of the state of nutrition vitamins in humans. water-soluble vitamins (C, biotin, niacin, thiamin, pantothenic acid, riboflavin, pyridoxine, folate, B12). Fat-soluble vitamins (A, E, K, D). Choline, carnitine, taurine. Coenzymes. Structure and function of biological membranes: membrane composition. structure and properties of membrane lipids. systems of transport through the membrane. structure and function of the Na + / K + ATPase. General aspects of metabolism: catabolism and anabolism. Bioenergetics: the general principles of chemical thermodynamics. Redox potential. links to high energy of hydrolysis. ATP: structure and role in the transfer of energy. Organization of metabolic pathways. the pyruvate dehydrogenase complex. Krebs cycle: metabolic role and regulation. respiratory chain and oxidative phosphorylation. Inhibitors of the respiratory chain and uncoupling agents

CHEMISTRY - MOD. 1

Code	50202503
Location	PERUGIA
CFU	3
Teacher	Pier Luigi Orvietani
Teachers	Pier Luigi Orvietani
Hours	37.5 ore - Pier Luigi Orvietani
Learning activities	Base
Area	Struttura, funzione e metabolismo delle molecole d'interesse biologico
Academic discipline	BIO/10
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	The electronic structure and the properties of atoms: the quantum numbers, the atomic orbitals, the electronic configurations of the elements Periodic table of the elements and the properties of elements: ionization potential, electron affinity and electronegativityCovalent molecules: the combination of s orbitals, the combination of p orbitals, the formation of diatomic molecules; poliatomic molecules.Chemical bonds: covalent bonds, ionic bonds, idrogen bond, ibridization, Inorganic moleculesSolid state, liquid state and vapor stateSolution chemistryAcids and bases and chemical balancepH measurements of different solutionsPrinciples of thermodynamics, chemical kinetics and oxidation status
Reference texts	Chimica e Propedeutica Biochimica - Luciano Binaglia e Bruno Giardina
Educational objectives	The main competence will be to have a general knowledge of inorganic chemistry
Prerequisites	In order to be able to understand and apply the majority of the contents described within the course, the student must have acquired basic knowledge of chemistry and mathematics studied for access to the Faculty of Medicine.
Teaching methods	The course is organized with face to face lessons on all subjects of the course using slide projections.Students are stimulated to interact with the teacher during the lectures.Hours available to the students to be established during the course for clarification and insights.During the course you will have an ongoing learning test
Other information	The attendance is compulsory at least 75% of lessonsTerni - School of Medicine - Educational and Scientific Center
Learning verification modality	The final exam consists of a pratical test and an oral exam
Extended program	The electronic structure and the properties of atoms: the quantum numbers, the atomic orbitals, the electronic configurations of the elementsPeriodic table of the elements and the properties of elements: ionization potential, electron affinity and electronegativityCovalent molecules: the combination of s orbitals, the combination of p orbitals, the formation of diatomic molecules; poliatomic molecules.Chemical bonds: covalent bonds, ionic bonds, idrogen bond, ibridization, Inorganic moleculesSolid state, liquid state and vapor stateSolution chemistryAcids and bases and chemical balancepH measurements of different solutionsPrinciples of thermodynamics, chemical kinetics and oxidation status

CHEMISTRY - MOD. 2

Code	50202002
Location	PERUGIA
CFU	2
Teacher	Pier Luigi Orvietani
Teachers	Pier Luigi Orvietani
Hours	25 ore - Pier Luigi Orvietani
Learning activities	Affine/integrativa
Area	Attività formative affini o integrative
Academic discipline	BIO/10
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Hydrocarbons: alkanes, alkenes, alkines and their characteristic reactionsaromatic hydrocarbons and their characteristic reactionsstereoisomery and enantiomersalcohols, phenols and their characteristic reactionsalkyl halidesaldehydes and ketones and reactions aliphatic and aromatic amines carboxylic acids and their reactions derivatives of carboxylic acidsaromatic heterocycles
Reference texts	Chimica e Propedeutica Biochimica - Luciano Binaglia e Bruno Giardina
Educational objectives	The main competence will be to have a general knowledge of organic chemistry in order to approach the study of biochemistry
Prerequisites	In order to be able to understand and apply the majority of the contents described within the course, the student must have acquired basic knowledge of Inorganic chemistry
Teaching methods	The course is organized with face to face lessons on all subjects of the course using slide projections.Students are stimulated to interact with the teacher during the lectures.Hours available to the students to be established during the course for clarification and insights.During the course you will have an ongoing learning test
Other information	The attendance is compulsory at least 75% of lessonsTerni - School of Medicine - Educational and Scientific Center
Learning verification modality	The final exam consists of a pratical test and an oral exam
Extended program	Hydrocarbons: alkanes, alkenes, alkines and their characteristic reactionsaromatic hydrocarbons and their characteristic reactionsstereoisomery and enantiomersalcohols, phenols and their characteristic reactionsalkyl halidesaldehydes and ketones and reactions aliphatic and aromatic amines carboxylic acids and their reactions derivatives of carboxylic acidsaromatic heterocycles