Unit LABORATORY MEDICINE
- Course
- Medicine and surgery
- Study-unit Code
- GP005676
- Location
- PERUGIA
- Curriculum
- In all curricula
- CFU
- 8
- Course Regulation
- Coorte 2023
- Offered
- 2025/26
- Type of study-unit
- Obbligatorio (Required)
- Type of learning activities
- Attività formativa integrata
CLINICAL BIOCHEMISTRY
| Code | A001682 |
|---|---|
| Location | PERUGIA |
| CFU | 2 |
| Learning activities | Caratterizzante |
| Area | Medicina di laboratorio e diagnostica integrata |
| Sector | BIO/12 |
| Type of study-unit | Obbligatorio (Required) |
Cognomi A-L
- CFU
- 2
- Teacher
- Paolo Gorello
- Teachers
- Paolo Gorello
- Hours
- 25 ore - Paolo Gorello
- Language of instruction
- Italian
- Contents
- - Generation of laboratory data and its critical interpretation.- Reliability of test results: precision, accuracy, sensitivity, specificity.- Quality management, predictive value and decision-making levels.- Units of measurement in Clinical Biochemistry, sources of preanalytical variability, reference values, Biological variability.- Principles and techniques for collecting, processing and storage of biological samples.- Methods used in Clinical Biochemistry:Centrifugation techniques, UV-Visible spectrophotometry and Spectrofluorimetry- Point-of-Care testing, Chemical dry state.- Clinical Enzymology.- Biochemical parameters. Markers of function and injury of tissue or organ with particular reference to markers of myocardial damage and markers of liver function. - Description of the most used techniques in Clinical Biochemistry: Nucleic acids electrophoresis, Protein electrophoresis, Western-blotting , ELISA assay, RIA assay, Fluorescence Microscopy.- Basic elements of Molecular Biology (genome, gene, mutations, polymorphisms) and utilization of databases (eg. NCBI, Enseble Genome Browser, Globin Gene Server).- Integration between Clinical Biochemistry methods and Molecular Biology methods : Study of Hemoglobinopathies- Description of the most used techniques in Molecular Diagnostics:end-point PCR , Real-Time PCR, dHPLC, DNA sequencing (Sanger method) , NGS (Next Generation Sequencing).
- Reference texts
- Slides and notes provided by teacher 1) M. Ciaccio, G. Lippi Biochimica Clinica E MEDICINA DI LABORATORIO Edises 2) I. Antonozzi, E. Gulletta MEDICINA DI LABORATORIO Logica e Patologia Clinica PICCIN
- Educational objectives
- The main aim of this teaching is to provide students with the theoretical principles useful to identify and analyze the sources of variability of laboratory data and to provide students with the theoretical principles needed to understand the applications and issues that underlie the methods most used in Clinical Biochemistry and Molecular Biology. The program includes the presentation of some investigation methods of laboratory, their application in the diagnosis of diseases. The main knowledge acquired will be:- Basic knowledge about the collection, processing and storage of biological samples.- Knowledge related to units of measurement in Clinical Biochemistry, sources of preanalytical variability, reference values, biological variability.- Basic concepts of reliability analytical precision, accuracy, sensitivity, specificity.- Basic elements about quality management , predictive value and decision-making levels.- Basic knowledge of Methods used in Clinical Biochemistry:Centrifugation techniques, UV-Visible spectrophotometry and spectrofluorimetry.- Basic knowledge of Clinical Enzymology: enzyme assay, markers of tissue and organ. - Basic knowledge of Methods used in Clinical Biochemistry: Nucleic acids and protein electrophoresis, Western-blotting, ELISA, RIA and TestFluorescence microscopy. - Basic knowledge of Methods used in Clinical Molecular Biology: End-point PCR, Real-Time PCR, dHPLC, DNA sequencing using Sanger method,NGS (Next Generation Sequencing). - Basic elements of Molecular Biology (genome, gene, mutations, polymorphisms) and utilization of databases (eg. NCBI, Enseble Genome Browser, Globin Gene Server).
- Prerequisites
- The course requires basic knowledge of Biochemistry, Cell Biology and Genetics.
- Teaching methods
- Lectures on all subjects of the couse.
- Other information
- Multiple choice written test: 30 questions relating to all aspects covered during the course (1 point for each question). FINAL MARK: arithmetic mean of the marks obtained in the individual disciplines (Clinical Microbiology, Clinical Pathology, Clinical Biochemistry). - Generation of laboratory data and its critical interpretation.- Reliability of test results: precision, accuracy, sensitivity, specificity.- Quality management, predictive value and decision-making levels.- Units of measurement in Clinical Biochemistry, sources of preanalytical variability, reference values, Biological variability.- Principles and techniques for collecting, processing and storage of biological samples.- Methods used in Clinical Biochemistry:Centrifugation techniques, UV-Visible spectrophotometry and Spectrofluorimetry- Point-of-Care testing, Chemical dry state.- Clinical Enzymology.- Biochemical parameters. Markers of function and injury of tissue or organ with particular reference to markers of myocardial damage and markers of liver function. - Description of the most used techniques in Clinical Biochemistry: Nucleic acids electrophoresis, Protein electrophoresis, Western-blotting , ELISA assay, RIA assay, Fluorescence Microscopy.- Basic elements of Molecular Biology (genome, gene, mutations, polymorphisms) and utilization of databases (eg. NCBI, Enseble Genome Browser, Globin Gene Server).- Integration between Clinical Biochemistry methods and Molecular Biology methods : Study of Hemoglobinopathies- Description of the most used techniques in Molecular Diagnostics:end-point PCR , Real-Time PCR, dHPLC, DNA sequencing (Sanger method) , NGS (Next Generation Sequencing).
- Extended program
- - Generation of laboratory data and its critical interpretation.- Reliability of test results: precision, accuracy, sensitivity, specificity.- Quality management, predictive value and decision-making levels.- Units of measurement in Clinical Biochemistry, sources of preanalytical variability, reference values, Biological variability.- Principles and techniques for collecting, processing and storage of biological samples.- Methods used in Clinical Biochemistry:Centrifugation techniques, UV-Visible spectrophotometry and Spectrofluorimetry- Point-of-Care testing, Chemical dry state.- Clinical Enzymology.- Biochemical parameters. Markers of function and injury of tissue or organ with particular reference to markers of myocardial damage and markers of liver function. - Description of the most used techniques in Clinical Biochemistry: Nucleic acids electrophoresis, Protein electrophoresis, Western-blotting , ELISA assay, RIA assay, Fluorescence Microscopy.- Basic elements of Molecular Biology (genome, gene, mutations, polymorphisms) and utilization of databases (eg. NCBI, Enseble Genome Browser, Globin Gene Server).- Integration between Clinical Biochemistry methods and Molecular Biology methods : Study of Hemoglobinopathies- Description of the most used techniques in Molecular Diagnostics:end-point PCR , Real-Time PCR, dHPLC, DNA sequencing (Sanger method) , NGS (Next Generation Sequencing).
- Obiettivi Agenda 2030 per lo sviluppo sostenibile
- Good Health and well-being
Cognomi M-Z
- CFU
- 2
- Teacher
- Paolo Gorello
- Teachers
- Paolo Gorello
- Hours
- 25 ore - Paolo Gorello
- Language of instruction
- Italian
- Contents
- - Generation of laboratory data and its critical interpretation.- Reliability of test results: precision, accuracy, sensitivity, specificity.- Quality management, predictive value and decision-making levels.- Units of measurement in Clinical Biochemistry, sources of preanalytical variability, reference values, Biological variability.- Principles and techniques for collecting, processing and storage of biological samples.- Methods used in Clinical Biochemistry:Centrifugation techniques, UV-Visible spectrophotometry and Spectrofluorimetry- Point-of-Care testing, Chemical dry state.- Clinical Enzymology.- Biochemical parameters. Markers of function and injury of tissue or organ with particular reference to markers of myocardial damage and markers of liver function. - Description of the most used techniques in Clinical Biochemistry: Nucleic acids electrophoresis, Protein electrophoresis, Western-blotting , ELISA assay, RIA assay, Fluorescence Microscopy.- Basic elements of Molecular Biology (genome, gene, mutations, polymorphisms) and utilization of databases (eg. NCBI, Enseble Genome Browser, Globin Gene Server).- Integration between Clinical Biochemistry methods and Molecular Biology methods : Study of Hemoglobinopathies- Description of the most used techniques in Molecular Diagnostics:end-point PCR , Real-Time PCR, dHPLC, DNA sequencing (Sanger method) , NGS (Next Generation Sequencing).
- Reference texts
- Slides and notes provided by teacher 1) M. Ciaccio, G. Lippi Biochimica Clinica E MEDICINA DI LABORATORIO Edises 2) I. Antonozzi, E. Gulletta MEDICINA DI LABORATORIO Logica e Patologia Clinica PICCIN
- Educational objectives
- The main aim of this teaching is to provide students with the theoretical principles useful to identify and analyze the sources of variability of laboratory data and to provide students with the theoretical principles needed to understand the applications and issues that underlie the methods most used in Clinical Biochemistry and Molecular Biology. The program includes the presentation of some investigation methods of laboratory, their application in the diagnosis of diseases. The main knowledge acquired will be:- Basic knowledge about the collection, processing and storage of biological samples.- Knowledge related to units of measurement in Clinical Biochemistry, sources of preanalytical variability, reference values, biological variability.- Basic concepts of reliability analytical precision, accuracy, sensitivity, specificity.- Basic elements about quality management , predictive value and decision-making levels.- Basic knowledge of Methods used in Clinical Biochemistry:Centrifugation techniques, UV-Visible spectrophotometry and spectrofluorimetry.- Basic knowledge of Clinical Enzymology: enzyme assay, markers of tissue and organ. - Basic knowledge of Methods used in Clinical Biochemistry: Nucleic acids and protein electrophoresis, Western-blotting, ELISA, RIA and TestFluorescence microscopy. - Basic knowledge of Methods used in Clinical Molecular Biology: End-point PCR, Real-Time PCR, dHPLC, DNA sequencing using Sanger method,NGS (Next Generation Sequencing). - Basic elements of Molecular Biology (genome, gene, mutations, polymorphisms) and utilization of databases (eg. NCBI, Enseble Genome Browser, Globin Gene Server).
- Prerequisites
- The course requires basic knowledge of Biochemistry, Cell Biology and Genetics.
- Teaching methods
- Lectures on all subjects of the couse.
- Other information
- Multiple choice written test: 30 questions relating to all aspects covered during the course (1 point for each question). FINAL MARK: arithmetic mean of the marks obtained in the individual disciplines (Clinical Microbiology, Clinical Pathology, Clinical Biochemistry). - Generation of laboratory data and its critical interpretation.- Reliability of test results: precision, accuracy, sensitivity, specificity.- Quality management, predictive value and decision-making levels.- Units of measurement in Clinical Biochemistry, sources of preanalytical variability, reference values, Biological variability.- Principles and techniques for collecting, processing and storage of biological samples.- Methods used in Clinical Biochemistry:Centrifugation techniques, UV-Visible spectrophotometry and Spectrofluorimetry- Point-of-Care testing, Chemical dry state.- Clinical Enzymology.- Biochemical parameters. Markers of function and injury of tissue or organ with particular reference to markers of myocardial damage and markers of liver function. - Description of the most used techniques in Clinical Biochemistry: Nucleic acids electrophoresis, Protein electrophoresis, Western-blotting , ELISA assay, RIA assay, Fluorescence Microscopy.- Basic elements of Molecular Biology (genome, gene, mutations, polymorphisms) and utilization of databases (eg. NCBI, Enseble Genome Browser, Globin Gene Server).- Integration between Clinical Biochemistry methods and Molecular Biology methods : Study of Hemoglobinopathies- Description of the most used techniques in Molecular Diagnostics:end-point PCR , Real-Time PCR, dHPLC, DNA sequencing (Sanger method) , NGS (Next Generation Sequencing).
- Extended program
- - Generation of laboratory data and its critical interpretation.- Reliability of test results: precision, accuracy, sensitivity, specificity.- Quality management, predictive value and decision-making levels.- Units of measurement in Clinical Biochemistry, sources of preanalytical variability, reference values, Biological variability.- Principles and techniques for collecting, processing and storage of biological samples.- Methods used in Clinical Biochemistry:Centrifugation techniques, UV-Visible spectrophotometry and Spectrofluorimetry- Point-of-Care testing, Chemical dry state.- Clinical Enzymology.- Biochemical parameters. Markers of function and injury of tissue or organ with particular reference to markers of myocardial damage and markers of liver function. - Description of the most used techniques in Clinical Biochemistry: Nucleic acids electrophoresis, Protein electrophoresis, Western-blotting , ELISA assay, RIA assay, Fluorescence Microscopy.- Basic elements of Molecular Biology (genome, gene, mutations, polymorphisms) and utilization of databases (eg. NCBI, Enseble Genome Browser, Globin Gene Server).- Integration between Clinical Biochemistry methods and Molecular Biology methods : Study of Hemoglobinopathies- Description of the most used techniques in Molecular Diagnostics:end-point PCR , Real-Time PCR, dHPLC, DNA sequencing (Sanger method) , NGS (Next Generation Sequencing).
- Obiettivi Agenda 2030 per lo sviluppo sostenibile
- Good Health and well-being
CLINICAL MICROBIOLOGY
| Code | A001681 |
|---|---|
| Location | PERUGIA |
| CFU | 2 |
| Learning activities | Caratterizzante |
| Area | Patologia generale e molecolare, immunopatologia, fisiopatologia generale, microbiologia e parassitologia |
| Sector | MED/07 |
| Type of study-unit | Obbligatorio (Required) |
Cognomi A-L
- CFU
- 2
- Teacher
- Antonella Mencacci
- Teachers
- Antonella Mencacci
- Hours
- 25 ore - Antonella Mencacci
- Language of instruction
- Italian
- Contents
- Organization and role of the Clinical Microbiology laboratory.
Microbiological diagnostics of organ/system and apparatus infections - Reference texts
- - Microbiologia Medica. Murray PR, Rosenthal LS, Pfaller MA. Edra Masson.
- Principi di Microbiologia Medica, Antonelli G, Clementi M, Pozzi G, Rossolini GM
- Power-point presentations of the face-to-face lessons - Educational objectives
- Knowledge of the various methodological approaches that can be used for the direct and indirect microbiological diagnosis of infectious diseases.
Knowledge of the correct microbiological diagnostic procedures for the etiological diagnosis of infections, with particular reference to the appropriateness of the required tests and the interpretation of the results of microbiological investigations.
• Acquire an adequate diagnostic approach with respect to the infection suspected on a clinical basis
• Knowing how to request microbiological tests
• Knowing how to read and interpret a report. - Prerequisites
- Passing exams in Microbiology and Clinical Biochemistry.
Basic knowledge of Anatomy, Physiology, General Pathology, Immunology. - Teaching methods
- face-to-face lessons and practical activities on the main diagnostic procedures described in the frontal lessons.
- Other information
- Professor receives 30 minutes after each lesson or by appointment made by e-mail.
- Learning verification modality
- ORAL EXAMINATION lasting about 15 min divided into 2 questions, 1 of which relating to the diagnosis of infections of a system/apparatus (see program) and 1 more technical relating to the meaning of a diagnostic technology. A third question on the minimum indispensable knowledge can be made in the case of an unsatisfactory answer to the two questions.
VERIFICATION OF CLINICAL SKILLS: interpretation of a microbiological/antibiogram report
INTERNSHIP ACTIVITY: A question about a microbiological technique or diagnostic process.
Final MARK: average of the marks relating to the individual courses - Extended program
- Organization of the Clinical Microbiology laboratory in diagnostic sectors.
Security level 2 and 3 laboratories.
Direct and indirect diagnosis of bacterial, viral, fungal, protozoal infectious diseases:
Microscopic and culture tests, molecular tests, serological tests, immunodiagnosis.
Antibiogram and detection of antibiotic resistance mechanisms of Gram positive and Gram negative bacteria (methicillin resistance, vancomycin resistance, inducible resistance to macrolides/lincosamides, ESBL, carbapenemase).
Microbiological diagnosis of bacterial, viral, fungal and protozoal infections of the following systems and systems: cardio-circulatory system, urinary system, central nervous system, respiratory system (upper and lower respiratory tract), genitourinary system, gastrointestinal system (notes), infections of wounds and pressure sores, bones and joints (outline). Nosocomial infections and MDRO.
Nosographic classification of each infectious disease (e.g.: sepsis, pneumonia, urinary tract infections, meningitis, septic arthritis, etc.) and of the most appropriate microbiological diagnostic approach. Formulation of the request for microbiological tests and correct reading/interpretation of the report. - Obiettivi Agenda 2030 per lo sviluppo sostenibile
- .
Cognomi M-Z
- CFU
- 2
- Teacher
- Antonella Mencacci
- Teachers
- Antonella Mencacci
- Hours
- 25 ore - Antonella Mencacci
- Language of instruction
- Italian
- Contents
- Organization and role of the Clinical Microbiology laboratory.
Microbiological diagnostics of organ/system and apparatus infections - Reference texts
- - Microbiologia Medica. Murray PR, Rosenthal LS, Pfaller MA. Edra Masson.
- Principi di Microbiologia Medica, Antonelli G, Clementi M, Pozzi G, Rossolini GM
- Power-point presentations of the face-to-face lessons - Educational objectives
- Knowledge of the various methodological approaches that can be used for the direct and indirect microbiological diagnosis of infectious diseases.
Knowledge of the correct microbiological diagnostic procedures for the etiological diagnosis of infections, with particular reference to the appropriateness of the required tests and the interpretation of the results of microbiological investigations.
• Acquire an adequate diagnostic approach with respect to the infection suspected on a clinical basis
• Knowing how to request microbiological tests
• Knowing how to read and interpret a report. - Prerequisites
- Passing exams in Microbiology and Clinical Biochemistry.
Basic knowledge of Anatomy, Physiology, General Pathology, Immunology. - Teaching methods
- face-to-face lessons and practical activities on the main diagnostic procedures described in the frontal lessons.
- Other information
- Professor receives 30 minutes after each lesson or by appointment made by e-mail.
- Learning verification modality
- ORAL EXAMINATION lasting about 15 min divided into 2 questions, 1 of which relating to the diagnosis of infections of a system/apparatus (see program) and 1 more technical relating to the meaning of a diagnostic technology. A third question on the minimum indispensable knowledge can be made in the case of an unsatisfactory answer to the two questions.
VERIFICATION OF CLINICAL SKILLS: interpretation of a microbiological/antibiogram report
INTERNSHIP ACTIVITY: A question about a microbiological technique or diagnostic process.
Final MARK: average of the marks relating to the individual courses - Extended program
- Organization of the Clinical Microbiology laboratory in diagnostic sectors.
Security level 2 and 3 laboratories.
Direct and indirect diagnosis of bacterial, viral, fungal, protozoal infectious diseases:
Microscopic and culture tests, molecular tests, serological tests, immunodiagnosis.
Antibiogram and detection of antibiotic resistance mechanisms of Gram positive and Gram negative bacteria (methicillin resistance, vancomycin resistance, inducible resistance to macrolides/lincosamides, ESBL, carbapenemase).
Microbiological diagnosis of bacterial, viral, fungal and protozoal infections of the following systems and systems: cardio-circulatory system, urinary system, central nervous system, respiratory system (upper and lower respiratory tract), genitourinary system, gastrointestinal system (notes), infections of wounds and pressure sores, bones and joints (outline). Nosocomial infections and MDRO.
Nosographic classification of each infectious disease (e.g.: sepsis, pneumonia, urinary tract infections, meningitis, septic arthritis, etc.) and of the most appropriate microbiological diagnostic approach. Formulation of the request for microbiological tests and correct reading/interpretation of the report. - Obiettivi Agenda 2030 per lo sviluppo sostenibile
- .
CLINICAL PATHOLOGY
| Code | GP005709 |
|---|---|
| Location | PERUGIA |
| CFU | 2 |
| Learning activities | Caratterizzante |
| Area | Medicina di laboratorio e diagnostica integrata |
| Sector | MED/05 |
| Type of study-unit | Obbligatorio (Required) |
Cognomi A-L
- CFU
- 2
- Teacher
- Stefano Brancorsini
- Teachers
- Stefano Brancorsini
- Hours
- 25 ore - Stefano Brancorsini
Cognomi M-Z
- CFU
- 2
- Teacher
- Stefano Brancorsini
- Teachers
- Stefano Brancorsini
- Hours
- 25 ore - Stefano Brancorsini
PROFESSIONALISING TRAINING IN CLINICAL MICROBIOLOGY
| Code | GP005715 |
|---|---|
| Location | PERUGIA |
| CFU | 1 |
| Learning activities | Altro |
| Area | Tirocini formativi e di orientamento |
| Sector | MED/07 |
| Type of study-unit | Obbligatorio (Required) |
Cognomi A-L
- CFU
- 1
- Teacher
- Antonella Mencacci
- Teachers
- Antonella Mencacci
- Hours
- 25 ore - Antonella Mencacci
- Language of instruction
- Italian
- Contents
- Diagnostic iter related to the microbiological diagnosis of infections.
- Reference texts
- Murray PR, Rosenthal KS, Pfaller MA. Microbiologia Medica, Edra Masson
Antonelli G, Clementi M, Pozzi G, Rossolini G.M. Principi di Microbiologia Medica, CEA - Educational objectives
- the student will have to know the diagnostic procedures, from the collection of the clinical sample to the final reporting of the tests, relating to the following infections: bloodstream infections, meingitis, respiratory infections, urinary tract infections, and skin and soft tissues infections.
- Prerequisites
- .
- Teaching methods
- Practical activity at the Microbiology Unit (Perugia General Hospital)
- Other information
- .
- Learning verification modality
- The same as Clinical Microbiology
- Extended program
- Diagnostic process for the diagnosis of:
sepsis, pneumonia, meningitis, urinary tract infections, skin and soft tissue infections. - Obiettivi Agenda 2030 per lo sviluppo sostenibile
- .
Cognomi M-Z
- CFU
- 1
- Teacher
- Antonella Mencacci
- Teachers
- Antonella Mencacci
- Hours
- 25 ore - Antonella Mencacci
- Language of instruction
- Italian
- Contents
- Diagnostic iter related to the microbiological diagnosis of infections.
- Reference texts
- Murray PR, Rosenthal KS, Pfaller MA. Microbiologia Medica, Edra Masson
Antonelli G, Clementi M, Pozzi G, Rossolini G.M. Principi di Microbiologia Medica, CEA - Educational objectives
- the student will have to know the diagnostic procedures, from the collection of the clinical sample to the final reporting of the tests, relating to the following infections: bloodstream infections, meingitis, respiratory infections, urinary tract infections, and skin and soft tissues infections.
- Prerequisites
- .
- Teaching methods
- Practical activity at the Microbiology Unit (Perugia General Hospital)
- Other information
- .
- Learning verification modality
- The same as Clinical Microbiology
- Extended program
- Diagnostic process for the diagnosis of:
sepsis, pneumonia, meningitis, urinary tract infections, skin and soft tissue infections. - Obiettivi Agenda 2030 per lo sviluppo sostenibile
- .
PROFESSIONALISING TRAINING IN CLINICAL PATHOLOGY
| Code | GP005714 |
|---|---|
| Location | PERUGIA |
| CFU | 1 |
| Learning activities | Altro |
| Area | Tirocini formativi e di orientamento |
| Sector | MED/05 |
| Type of study-unit | Obbligatorio (Required) |
Cognomi A-L
- CFU
- 1
- Teacher
- Stefano Brancorsini
- Teachers
- Stefano Brancorsini
- Hours
- 25 ore - Stefano Brancorsini
Cognomi M-Z
- CFU
- 1
- Teacher
- Stefano Brancorsini
- Teachers
- Stefano Brancorsini
- Hours
- 25 ore - Stefano Brancorsini