Unit BIOCHEMICAL BIOTECHNOLOGY FOR MOLECULAR ASSAYS WITH LABORATORY

BIOCHEMICAL BIOTECHNOLOGY FOR MOLECULAR ASSAYS

Code	A004631
CFU	1
Teacher	Sabata Martino
Teachers	Sabata Martino
Hours	7 ore - Sabata Martino
Learning activities	Affine/integrativa
Area	Attività formative affini o integrative
Sector	BIO/10
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	L’insegnamento fornisce competenze teoriche e pratiche per la progettazione, esecuzione, validazione e interpretazione di saggi molecolari biochimici applicati in ambito diagnostico, industriale, ambientale e biotecnologico.
Reference texts	Slide del docente Protocolli di laboratorio forniti
Educational objectives	Al termine del corso lo studente sarà in grado di: Comprendere i principi biochimici dei principali saggi molecolari. Progettare un saggio molecolare in funzione dell’obiettivo applicativo. Utilizzare correttamente strumentazione di laboratorio per analisi molecolari. Analizzare criticamente dati sperimentali e redigere un report tecnico. Applicare criteri di qualità, riproducibilità e controllo (GLP). Operare in sicurezza in laboratorio biotecnologic
Prerequisites	Biochimica di base Biologia molecolare Chimica generale
Teaching methods	Lezioni frontali con casi applicativi reali Didattica laboratoriale hands-on Problem solving guidato Analisi dati con foglio elettronico Discussione errori sperimentali
Learning verification modality	Esame scritto individuale . Consisterà in 30 domande a risposta multipla. 15 domande riguarderanno le lezioni teoriche, le altre 15 domande riguarderanno l'attività di laboratorio.
Extended program	Lezioni Frontali: Classificazione dei saggi molecolari biochimici; Saggi qualitativi vs quantitativi; Concetto di target molecolare; Parametri analitici (accuratezza, precisione, sensibilità); ELISA: principi e varianti; Saggi enzimatici accoppiati; Rivelazione colorimetrica, fluorimetrica, chemiluminescente; Applicazioni in ambito clinico e industriale; Cenni su: PCR convenzionale e qPCR; Sonde fluorescenti; Quantificazione assoluta e relativa; Contaminazioni e controlli; Cenni su: Controllo qualità e GLP Tracciabilità e gestione del dato Applicazioni in: Diagnostica molecolare, Sicurezza alimentare, Monitoraggio ambientale, Controllo qualità industriale Attività di laboratorio: – Tecniche di base e quantificazione proteica: allineamento su tecniche di base (Uso corretto di micropipette e bilance analitiche; preparazione soluzioni tampone; Curva standard (Bradford ) Analisi spettrofotometrica; Elaborazione dati e costruzione curva di calibrazione -Saggio immunoenzimatico (ELISA): Preparazione piastra; Incubazioni e lavaggi; Rivelazione colorimetrica; Lettura spettrofotometrica Calcolo concentrazione campioni incogniti -saggio enzimatico

BIOCHEMICAL MOLECULAR DIGNOSTIC ASSAYS

Code	A004814
CFU	1
Teacher	Paolo Gorello
Teachers	Paolo Gorello
Hours	7 ore - Paolo Gorello
Learning activities	Affine/integrativa
Area	Attività formative affini o integrative
Sector	BIO/12
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	Selection of biological samples ( DNA, RNA and Proteins) and quality control analysis. PCR: Theoretical bases and set-up of PCR reactions. Database: nucleotide and amino acid sequences. Real-Time PCR: Theoretical bases, Fluorogenic probes, endogenous housekeeping genes. DNA sequencing and mutational analysis : Sanger method , NGS (Next Generation Sequencing) and Clinical applications.
Reference texts	Slides and notes provided by teacher
Educational objectives	The main aim of this teaching is to provide students with the theoretical principles that are the basis of the most common techniques of molecular biology, in order to identify possible applications in diagnosis.
Prerequisites	The course requires basic knowledge of Biochemistry, Cell Biology and Genetics.
Teaching methods	Lectures on all subjects of the couse.
Learning verification modality	Written Test
Extended program	Selection of biological samples ( DNA, RNA and Proteins) and quality control analysis. PCR: Theoretical bases and set-up of PCR reactions. Database: nucleotide and amino acid sequences. Real-Time PCR: Theoretical bases, Fluorogenic probes, endogenous housekeeping genes. DNA sequencing and mutational analysis : Sanger method , NGS (Next Generation Sequencing) and Clinical applications.
Obiettivi Agenda 2030 per lo sviluppo sostenibile	Good Health and well-being

LABORATORY OF BIOCHEMICAL BIOTECHNOLOGY FOR MOLECULAR ASSAYS IN DIAGNOSTICS

Code	A004776
CFU	2
Teacher	Paolo Gorello
Teachers	Paolo Gorello
Hours	24 ore - Paolo Gorello
Learning activities	Altro
Area	Altre conoscenze utili per l'inserimento nel mondo del lavoro
Sector	NN
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	PCR ELETTROFORESI DATABASE
Reference texts	Slides and notes provided by teacher
Educational objectives	L'obiettivo principale dell' insegnamento è quello di fornire agli studenti i principi teorici che sono alla base delle più comuni tecniche di biologia molecolare, allo scopo di identificare le possibili applicazioni in ambito diagnostico.
Prerequisites	The course requires basic knowledge of Biochemistry, Cell Biology and Genetics.
Teaching methods	Laboratory
Extended program	PCR, Electrophoresis, DATABASE
Obiettivi Agenda 2030 per lo sviluppo sostenibile	Good Health and well-being

LABORATORY FOR BIOCHEMICAL BIOTECHNOLOGIES FOR APPLIED MOLECULAR ASSAYS

Code	A004777
CFU	2
Teacher	Sabata Martino
Teachers	Sabata Martino
Hours	24 ore - Sabata Martino
Learning activities	Altro
Area	Altre conoscenze utili per l'inserimento nel mondo del lavoro
Sector	NN
Type of study-unit	Obbligatorio (Required)
Language of instruction	Italian
Contents	The course aims to provide students with knowledge of the main methods and technologies associated with biochemistry in the context of biotechnological research and in diagnostic and therapeutic applications. The course also includes a part of practical exercises on related topics (see BIOCHEMICAL BIOTECHNOLOGIES FOR APPLIED MOLECULAR ASSAYS)
Reference texts	Mauro Maccarrone Biochemical and biomolecular methodologies Ed. Zanichelli. Teaching material provided by the teacher.
Educational objectives	At the end of the course the student will have acquired the theoretical and practical knowledge of biochemical methods necessary to work effectively in a laboratory where relevant techniques are used.
Prerequisites	Basic knowledge of Organic Chemistry and Biochemistry
Teaching methods	l practical lessons in the teaching laboratory relating to the topics that are provided in the course of BIOCHEMICAL BIOTECHNOLOGIES FOR MOLECULAR ASSAYS.
Other information	For teaching activities (dates and times of lectures, classroom), please refer to website dcbb.unipg.it and the course page on Unistudium
Learning verification modality	The course provides basic laboratory skills aimed at improving skills in the health and environmental sectors.
Extended program	The course program includes practical exercises regarding the preparation of buffer solutions of biochemical interest, with the acquisition of skills regarding stoichiometric calculations, dilutions and percentages; ii) the use of buffer systems of biological interest and the measurement of pH; preparation of protein extracts or nucleic acids Determination of proteins and nucleic acids. Enzyme assays. PCR. ELISA assays. Use of specialized instrumentation for the determination of proteins and nucleic acids.
Obiettivi Agenda 2030 per lo sviluppo sostenibile	The course provides basic laboratory skills aimed at improving skills in the health and environmental sectors.