| Code |
A004613 |
| CFU |
6 |
| Teacher |
Sandra Buratta |
| Teachers |
|
| Hours |
|
| Learning activities |
Affine/integrativa |
| Area |
Attività formative affini o integrative |
| Sector |
BIOS-07/A |
| Type of study-unit |
Obbligatorio (Required) |
| Language of instruction |
Italian |
| Contents |
Structure and function of nucleic acids. Amino acids. Structure and function of proteins. Myoglobin and hemoglobin. Enzymes: catalysis and kinetics. Carbohydrates: structure and functions. Lipids: structure and function. Biological membranes. Introduction to metabolism. Carbohydrate metabolism. Tricarboxylic acid cycle. Electron transport chain and oxidative phosphorylation. |
| Reference texts |
1. Introduzione alla Biochimica di Lehninger di David L. Nelson, Michael, M. Cox. Ed. Zanichelli |
| Educational objectives |
At the end of the course the student will have to know: structures and properties of the main biological macromolecules (carbohydrates, lipids, proteins, DNA); their modes of interaction, the main metabolic and energy production pathways of the cell. |
| Prerequisites |
In order to understand the issues of the course, the students must have good basic knowledge in the field of general and inorganic chemistry and organic chemistry. Regarding biological aspects, students must have basic knowledge acquired during their secondary school. Further knowledge will be acquired by the students during the course. |
| Teaching methods |
The course will be organized with. Lessons will be integrated with slide |
| Other information |
Attending classes: recommended but not compulsory |
| Learning verification modality |
The exam consists of a written test designed to evaluate the level of knowledge and understanding of the topics covered in the course. The test has a maximum duration of two hours and consists of forty multiple-choice questions. |
| Extended program |
Amino acids, peptides, and proteins. Secondary, tertiary, and quaternary structure of proteins. Enzymes: catalytic mechanism; enzyme kinetics; mechanisms of inhibition and regulation of enzymatic activity. Carbohydrates: monosaccharides, disaccharides, polysaccharides, glycoconjugates. Nucleotides and nucleic acids: basic concepts of structure and function. Lipids: structure and function of the main classes of structural and storage lipids. Biological membranes: components, structure, and transport mechanisms. Principles of bioenergetics and metabolism: glycolysis and fermentations; citric acid cycle; fatty acid catabolism; principles of bioenergetics and biological redox reactions; electron transport chain and oxidative phosphorylation. |
| Code |
55056103 |
| CFU |
3 |
| Teacher |
Sabata Martino |
| Teachers |
|
| Hours |
|
| Learning activities |
Altro |
| Area |
Altre conoscenze utili per l'inserimento nel mondo del lavoro |
| Sector |
NN |
| Type of study-unit |
Obbligatorio (Required) |
| Language of instruction |
Italian |
| Contents |
Practical activities regarding thepreparation of buffer and solutions extraction of macromolecules (proteins, lipids, nucleic acids) from biological matrices |
| Reference texts |
Protocols provided by the teacher |
| Educational objectives |
The laboratory activities on methods for extracting the main classes of biological molecules will enable students to acquire the fundamental approaches and practices commonly used in a biochemistry laboratory. |
| Prerequisites |
To better understand the laboratory activities related to the topics covered in the program, the student must have a basic knowledge of biology. |
| Teaching methods |
The course is organized into practical activities focused on the preparation of buffer solutions, pH determination, and precipitation and extraction of biological molecules using organic solvents |
| Other information |
For teaching activities (dates and times of lectures, classroom), please refer to website dcbb.unipg.it and the course page on Unistudium |
| Learning verification modality |
The exam consists in written test. For information on support services for students with disabilities and / or DSA visit the page |
| Extended program |
Preparation of buffer solutions of biochemical interest, with the acquisition of skills regarding stoichiometric calculations, dilutions, and percentages; ii) the use of buffer systems of biological interest and the measurement of pH; the preparation of cellular and tissue extracts; setup of calibration curves for the quantification of macromolecules using the spectrophotometer. |
| Obiettivi Agenda 2030 per lo sviluppo sostenibile |
The course provides basic laboratory skills aimed at improving competency in the fields of health and the environment. |
