Unit GENETIC AND MOLECULAR BASIS OF BIOPHARMACEUTICALS
- Course
- Pharmaceutical biotechnologies
- Study-unit Code
- A006276
- Curriculum
- In all curricula
- Teacher
- Marco Gargaro
- Teachers
-
- Marco Gargaro
- Hours
- 57 ore - Marco Gargaro
- CFU
- 6
- Course Regulation
- Coorte 2026
- Offered
- 2026/27
- Learning activities
- Caratterizzante
- Area
- Discipline biotecnologiche comuni
- Sector
- BIOS-14/A
- Type of study-unit
- Obbligatorio (Required)
- Type of learning activities
- Attività formativa monodisciplinare
- Language of instruction
- English
- Contents
- The course consists of lectures and practical exercises aimed at learning genetic engineering techniques for the production of new biotechnological drugs such as recombinant proteins and enzymes.
- Reference texts
- R.J. Reece- Analisi dei geni e dei genomi. EdiSES.
- Educational objectives
- The course of "Genetic and molecular basis of biodrugs" aims at giving to the students the skills to design and realize a biodrug. Theoretical classes will give the fundamental notions to identify potential new biodrugs and to modify physiologic proteins for a proper customization of the therapy. Laboratory classes aims at giving the skills for cloning, translating and verifying the biologic activity of a therapeutic fusion protein.
- Prerequisites
- Basic knowledge of biology, molecular biology, immunology, and pharmacology.
- Teaching methods
- Theorical and practical lessons.
- Other information
- Frequency required.
- Learning verification modality
- The acquisition of all the described skills will be verified by means of an oral examination. Oral test consists of an interview of about 30 minutes aiming to ascertain the knowledge and understanding level acquired by the student on theoretical and methodological contents as indicated on the program. Furthermore, the ability to perform mathematical calculations aimed at performing laboratory procedures will be also evaluated.
Students with disabilities and/or DSA are invited to visit the page dedicated to the tools and measures envisaged and to agree in advance what is necessary with the teacher (https://www.unipg.it/disabilita-e -dsa). - Extended program
- Theoretical lessons: Biodrugs, modifications of natural proteins for the development of biodrugs. New genetic engineering approaches for the design and development of biodrugs: CRISPR/Cas9. Systems to produce recombinant proteins in prokaryotes, insects, yeasts, and eukaryotes. Conventional, retroviral, and viral plasmid vectors. Biodrugs as drug therapy for autoimmune, cardiovascular, inflammatory and tumor diseases treatment.
Practical lessons: Snapgene and DNASTAR genomics: Software for biodrugs virtual design. Advanced technologies for biomolecules DNA cloning: Gibson assembly, In-Fusion Cloning. Molecular identification of single nucleotide polymorphisms and site-specific mutagenesis. Cloning, purification and functional analysis of the CTLA-4-Ig fusion protein. - Obiettivi Agenda 2030 per lo sviluppo sostenibile
- Health and wellness